Fig. 3.

Circadian oscillation of AQP5 and ANO1 expression in the rat SGs under DD conditions by Western blot analysis. Protein expression characterization (A) and relative levels of AQP5 (B) and ANO1 (C) at 6 h intervals in CT. The expression level was normalized with ß-ACTIN. For each time point, SG samples were loaded at 10 μg total protein per lane. Markers at band indicate relative mass (in kDa). The relative levels are displayed as the mean ± SD of five replicates per time-point (n = 5). P-values were calculated by one-way ANOVA and results were considered significant at p < 0.05. The Bonferroni test for post hoc comparisons was performed and p < 0.01 were considered significant differences compare to CT0 are indicated as ‘*’. Non-adjusted and sliced images are shown in supplementary Fig. 1.