Table 1.
Primer sequences for sqPCR.
| Gene Name | 5′-sequence-3′ | GenBank Number | |
|---|---|---|---|
| β-actin | Forward | GGAGATTACTGCCCTGGCTCCTA | NM_031144.3 |
| Reverse | GACTCATCGTACTCCTGCTTGCTG | ||
| Bmal1 | Forward | TTCATGAACCCGTGGACCAA | NM_024362.2 |
| Reverse | CCCTGGAATGCCTGGAACA | ||
| Per2 | Forward | TCTCAGAGTTTGTGCGATGATTTG | NM_031678.1 |
| Reverse | CACTGGGTGAAGGTACGTTTGG | ||
| Clock | Forward | ACACAGCCAGCGATGTCTCAA | NM_021856.1 |
| Reverse | CATGGCTCCTAACTGAGCTGAAAG | ||
| Cry1 | Forward | CGGCGACCTATGGATCAGTTG | NM_198750.2 |
| Reverse | TCCCAGCATTGATGCTCCAG | ||
| Ano1 | Forward | TCAAAGGCCGGTTTGTTGGTCG | NM_001107564.1 |
| Reverse | GGCGAAGGGTTCGAGGTTGAAG | ||
| Aqp5 | Forward | GCCGTCAATGCGCTGAACAAC | NM_012779.1 |
| Reverse | CATGGAACAGCCGGTGAAGTAGATC |
Bmal1, aryl hydrocarbon receptor nuclear translocator-like protein 1; Per2, period 2; Clock, circadian locomotor output cycles kaput; Cry1, cryptochrome circadian clock 1; Ano1, Anoctamin 1; Aqp5, Aquaporin 5; GenBank Number; the Accession number of NIH genetic sequence database.