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. 2019 Nov 29;6:430. doi: 10.3389/fvets.2019.00430

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Copyright © 2019 Cassmann, Moore, Smith and Greenlee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blots of brain samples from sheep that were inoculated intracranially with the bovine passaged TME agent (bTME). The blots were immunolabeled with either the Sha31 (top) or P4 (bottom) antibody. Sha31, The antibody Sha31 was used on brain homogenates from sheep with the VRQ/ARQ genotype. The banding patterns are similar to the inoculum (bTME). P4, The antibody P4 is immunoreactive with brain homogenates from sheep with the ARQ/ARQ genotype. The inoculum, bTME, does not bind with the P4 antibody.