Table 1.
Proteins identified in the high mobility group box B 1 (HMGB1) yeast 2-hybrid (Y2H) approach interactome in PC-3 cells.
| Gene (Aliases) | Uniprot Code | N | A | Biological Function | Previous References to Prostate Cancer (PCa) |
|---|---|---|---|---|---|
| DLAT (DLTA) | P10515 | 1 | 221–420 | Dihydrolipoamide-acetyltransferase (DLAT) in pyruvate dehydrogenase complex control of mitochondrial energetic metabolism [27]. | Enzymatic activity at the basal level is significantly higher in prostate cancer cells compared to benign prostate cells [28]. |
| DNAAF2 (KTU) | Q9NVR5 | 1 | 436–621 | Cytoskeletal component [29]. | Not previously reported |
| HDLBP (HBP, VGL) | Q00341 | 1 | 152–374 | HDLBP drives cell proliferation [30]. | Not previously reported |
| HOXA10 (HOX1H) | P31260 | 1 | * | Transcriptional control | HoxA10 is highly expressed in PCa cells and tissues and is involved in cancer cell proliferation [31,32]. |
| KRT7 (SCL) | P08729 | 11 | 109–301 | Cytoskeletal component | KRT7 circulating mRNA was identified in blood samples from a cohort of metastatic PCa patients [33]. |
| MNAT1 | P51948 | 1 | * | MAT1, encoded by MNAT1, binds to p53 and mediates p53 ubiquitin-degradation through MDM2, increases cell growth, and decreases cell apoptosis [34]. | Not previously reported |
| SPIN1 | Q9Y657 | 1 | 130–337 | Chromatin reader; promotes the expression of rRNA [35] | Not previously reported |
| UBE2E3 | Q969T4 | 5 | 25–111 | Control of transcription factor activity [36]. | Not previously reported |
| UBXN1 (SAKS1) | Q04323 | 1 | 57–238 | NF-κB can be negatively regulated by UBXN1 [37]. | Not previously reported |
| UHRF2 (NIRF, RNF107) | Q96PU4 | 4 | 157–284 | UHRF2 encodes a nuclear protein involved in cell-cycle regulation, and it is an important mediator of E2F1-induced cell death [38]. | Not previously reported |
| ZNF428 (C19orf37) | Q96B54 | 1 | 109–188 | Unknown | Not previously reported |
N: redundancy in clone isolation; A: Sequenced region in clones, Aa relative to ATG; * noncoding sequence.