Table 3.
Proteins identified in the HMGB1 Y2H interactome in primary tumor adenocarcinoma.
| Gene (Aliases) | Uniprot Code | N | A | Biological Function | Previous References to Prostate Cancer (PCa) |
|---|---|---|---|---|---|
| c-FOS | P01100 | 2 | 27–184 | Transcriptional regulation and control of cell growth and apoptosis. [44]. | Expression is elevated in the prostate upon castration-mediated androgen withdrawal [44]. |
| GOLM1 | Q8NBJ4 | 1 | 236–376 | PI3K-AKT-mTOR signaling [45]. | Upregulated in PCa has oncogenic functions [45]. |
| HNRNPU | Q00839 | 1 | 91–296 | DNA and RNA binding [46]. | Not previously reported |
| MAP1B | P46821 | 2 | 2187–2409 | Vesicle formation; it can interact with p53 [47]. | Not previously reported |
| MAPKAPK5 | Q8IW41 | 1 | 1–95 | Involved in mTOR signaling [48]; MAPKAPK5 has diverse roles in cell growth, programmed cell death, senescence, and motility [49]. | Not previously reported |
| MIEN1 | Q9BRT3 | 3 | 24–204 | Regulator of cell migration and invasion [50]. | MIEN1 increases invasive potential of PCa cells by NF-κβ-mediated downstream target genes [50]. |
| MT2A | P02795 | 1 | 8–61 | Binding to heavy metals [51]. | MT2A is upregulated under hypoxia in PCa cell lines, PCa tissue, and residual cancer cells after androgen ablation therapy [52]. |
| PSMA7 (PTPT) | O14818 | 1 | 173–248 | PSMA7, a proteasome subunit, enhances AR transactivation in a dose-dependent manner [53] and inhibits the transactivation function of HIF-1A [54]. | Proposed biomarker in PCa [55] |
| PTPN2 | P17706 | 3 | 1–221 | Tyrosine-specific phosphatase (TCPTP) negatively regulates STAT3 that is involved in cell growth and proliferation, differentiation, migration, and cell death or apoptosis [56]. | Not previously reported |
| RASAL2 | Q9UJF2 | 1 | 97–334 | Tumor suppressor via RAS [57] | Not previously reported |
| RSF1 | Q96T23 | 1 | 572–795 | Chromatin remodeling factor necessary for p53-dependent gene expression in response to DNA damage [58]. | RSF1 is overexpressed in PCa and contributes to prostate cancer cell growth and invasion [59]. |
| SRSF3 | P84103 | 2 | 1–164 | Oncogenic splicing factor [60]. | SRSF3 expression is induced by hypoxia in prostate cancerous cells [61]. |
| TAF3 | Q5VWG9 | 5 | 2–222 | Transcriptional regulation; interacts with and inhibits p53 [62]. | Not previously reported |
| TGM3 | Q08188 | 1 | 480–693 | Catalyze the irreversible cross-linking of peptide-bound glutamine residues to lysines or primary amines; involved in apoptosis [63]. | Not previously reported |
| UBC | P0CG48 | 1 | 28–181 | Unanchored-polyubiquitin has several roles in activation of protein kinases, and signaling | Not previously reported |
| WNK4 | Q96J92 | 4 | 9–208 | Regulates STE20-related protein kinases that function upstream of the MAPK pathways. [64]. | Not previously reported |
| YY1 | P25490 | 1 | 27–223 | Transcriptional regulation [65]. | Involved in PCa [65,66,67,68,69,70] |
| ZNF428 | Q96B54 | 2 | 89–188 | Unknown | Not previously reported |
N: redundancy in clone isolation; A: Sequenced region in clones, Aa relative to ATG.