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. 2019 Oct 29;11(11):1684. doi: 10.3390/cancers11111684

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of miR-25-5p inhibitor on colony formation, cell viability, and apoptosis-related proteins in MLT- and/or Ptero-treated colon cancer cells. Effect of miR-25-5p inhibitor on the colony formation of HT29 (A) and HCT116 (B) cells treated with MLT (1mM) and/or Ptero (40µM). Colony formation assay was conducted in HT29 and HCT116 cells transfected with miR-25-5p inhibitor plasmid compared to intact control cells for 2 weeks. (C) Effect of miR-25-5p inhibitor on the cytotoxicity of MLT+Ptero in HT29, SW480, and HCT116 cells. The miRNA inhibitor control and miR-25-5p inhibitor plasmids were transfected into three colon cancer cells for 48h and then exposed to MLT and/or Ptero for 24 h. Cell viability was determined by MTT assay. ** p < 0.01, *** p < 0.001 vs. miRNA inhibitor negative control. Data represent means ± SEM of triplicate samples. (D) Effect of miR-25-5p inhibitor on PARP cleavage and Bcl-xL in HT29 and SW480 cells treated with MLT and/or Ptero.