Table 1.
Spectral regions used for analytical interpretation for Stroke study.
| Infrared Band Assignment | Spectral Range (cm−1) | Comments | |
|---|---|---|---|
| Amide I + II | 1700–1500 | Total protein region | |
| Protein components | Amide I | 1700–1600 | Specifically sensitive to protein secondary structure |
| Amide II | 1555–1535 | Mainly ν(C–N) associated with proteins | |
| β-sheet | 1610–1635 | Functional protein, protein secondary structure | |
| α-helix | 1660–1650 | Functional protein, protein secondary structure | |
| Random coil | 1645–1630 | protein secondary structure | |
| C-H region | 3100–2800 | Total lipid region | |
| Lipid component | νsCH2 | 2852–2800 | to measure lipid acyl chain length |
| νasCH2 | 2915–2930 | ||
| νasCH3 | 2950–2960 | to measure concentration of methyl content | |
| ν (C = O) | 1755–1715 | to measure oxidative stress | |
| ν (olefinic = CH) | 3000–3027 | to measure unsaturated lipid |
ν = stretching vibration; νas = asymmetric stretch; νs = symmetric stretch. * Resolutions Pro data analyzing software (V5.0), Cytospec (V2.00.03) Origin Lab (2019), Matlab (V2018b) and Cytospec (2.00.06) were used intensively to process all spectra and spectral images.