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. 2019 Oct 25;11(11):1654. doi: 10.3390/cancers11111654

Figure 1.

Figure 1

Figure 1

The simultaneous inhibition of CHK1/CHK2 and WEE1 synergizes in term of reduction of cell viability and induction of apoptosis in ALL cell lines. (A) Cell viability of RPMI-8402 and NALM-6 cell lines treated simultaneously with increasing concentration of PF-00477736 (nM) and AZD-1775 (nM) for 24, 48 and 72 h. Abbreviations PF = PF-00477736, AZD = AZD-1775. (B) Growth curve analysis of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM; dark grey full square) and PF-00477736 (25 and 250 nM respectively; light gray full triangle). In the graph AZD-1775 in combination with PF-00477736 is named AZD + PF(black full triangle). (C) Apoptosis analysis on RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). In the figures statistical significance was represented as asterisks and in detail: p < 0.05 one asterisk (*); p < 0.01 two asterisks (**); p < 0.001 three asterisks (***).