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. 2019 Nov 8;11(11):1762. doi: 10.3390/cancers11111762

Figure 6.

Figure 6

Depletion of DOCK1 transcriptionally upregulates the CLDN and tight-junction protein gene expression. (A,B) Claudin-low breast cancer cells were treated with shDOCK1 for three days. Collected cells were used for gene expression analysis by qRT-PCR analysis. The levels shown by qRT-PCR analysis were normalized to the level of β-actin (ACTB) and were expressed as the mean ± SE of three independent experiments. * p < 0.05; ** p < 0.01, compared with the control (shLuc). (C) Claudin-low breast cancer cells treated with shDOCK1 for 24 h and then with 1 μM actinomycin D (ActD) for 48 h were used for Western blot analysis. (D) Claudin-low breast cancer cells transfected with the pGL4-Basic or pGL4-Claudin-1 vector for 24 h and then with shDOCK1 for two days were used for the promoter activity assay. ** p < 0.01, compared with the control (Void).