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. 2019 Nov 13;11(11):1785. doi: 10.3390/cancers11111785

Figure 2.

Figure 2

Overexpression of KDM4C hinders acinar morphogenesis of RWPE-1 cells. (A) Western blotting assay confirming the overexpression of KDM4C in RWPE-1 cells. Molecular weight label of 130 and 110 kDa was shown. (B) Confocal images of control RWPE-1 cells (mock) and RWPE-1 cells overexpressing KDM4C forming organoids in 3D culture. Images were taken on the 2nd and 6th day. The structures were immune-stained with basal extracellular membrane receptor α6-integrin (green) and the apical marker GM130 (red). Nuclei were counterstained with DAPI dye (blue).