Figure 4.

Micro-Western array (MWA) and Western blotting analysis indicated that knockdown of KDM4C suppressed AKT signaling and c-Myc in PCa cells. (A) Expression of proteins involved in cell cycle regulation, EGFR signaling, AKT signaling, Src signaling, and JNK signaling in LNCaP C4-2B cells with or without KDM4C siRNA KD was analyzed with MWA. (B) Expression level of proteins with a fold change larger than 2.0 or smaller than 0.5 between control and KDM4C knockdown analyzed from (A) is demonstrated in a heatmap with the value of the protein expression level being converted to log₂. (C) Western blotting assay was used to confirm the change in proteins observed by MWA. Expression of KDM4C, mTOR, PTEN, PDK1, phospho-PDK1 S241, c-Myc, AKT, phospho-AKT T308 and phospho-AKT S473 was determined in triplicates of control LNCaP C4-2B cells and LNCaP C4-2B cells with KDM4C knockdown. β-Actin was used as the loading control.