Skip to main content
. 2019 Nov 13;11(11):1785. doi: 10.3390/cancers11111785

Figure 5.

Figure 5

KDM4C, AR, and c-Myc co-localized in the nuclei of PCa cells and overexpression of c-Myc or AKT rescued the suppressive effect of KDM4C knockdown on the proliferation of PCa cells. (A) Immunofluorescent microscopy revealed that KDM4C co-localized with AR and c-Myc in the nuclei of LNCaP C4-2B cells. The green bar in each image represents 10 μm. (B) Western blotting confirmed the KDM4C KD and overexpression of c-Myc. (C) Proliferation of control LNCaP C4-2B cells, LNCaP C4-2B with KDM4C KD, and LNCaP C4-2B with KDM4C KD plus c-Myc overexpression was determined by a Hoechst-dye 96 well proliferation assay for 48 h. (D) Western blotting confirmed KDM4C KD and overexpression of AKT. (E) Proliferation of control C4-2B cells, C4-2B with KDM4C KD, and C4-2B with KDM4C KD plus AKT overexpression was determined by Hoechst-dye 96 well proliferation assay for 48 h. Asterisks * and ** represented statistical significance, p < 0.05 and p < 0.01, respectively, among the two groups being compared.