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. 2019 Nov 14;10(11):930. doi: 10.3390/genes10110930

Figure 1.

Figure 1

Schematic of vector construction and thin layer chromatography (TLC) analysis of nicotine in wild-type and transgenic tobacco. (a) Schematic of the binary vector construction used for overexpression in tobacco. (b) TLC assay for the detection of nicotine accumulation in the leaves of 5-week-old wild-type and the NtERF32, NtERF221, or NtMYC2a overexpression lines. The plantlets were treated with 0.1% DMSO (control) or 100 µM methyljasmonic acid (MeJA) for 48 h before the leaf tissue was collected for the alkaloid extraction. Arrows indicate nicotine bands, and arrowheads indicate quinaldine as internal control.