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. 2019 Nov 14;10(11):930. doi: 10.3390/genes10110930

Figure 2.

Figure 2

Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) verification of the transcript levels of NtERF32, NtERF221, and NtMYC2a in wild-type and transgenic tobacco. Two-week-old wild-type or T2 generation seedlings of NtERF32, NtERF221 and NtMYC2a overexpression lines were treated with 0.1% DMSO (control) or 100 µM MeJA for 8 h before being collected for total RNA extraction and qRT-PCR experiment. Expression level was represented as mean of relative expression values from three biological replicates (n = 3) normalized to N. tabacum Elongation Factor 1-alpha (NtEF-1α). Error bars indicate standard error. Colored asterisks indicate the statistical significance of the expression values relative to the wild-type controls with unpaired t-tests at p < 0.01.