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. 2019 Nov 14;10(11):930. doi: 10.3390/genes10110930

Figure 4.

Figure 4

Expression levels of the structural genes that were upregulated by NtERF221 in wild-type and transgenic tobacco. Two-week-old wild-type or the transgenic tobacco seedlings overexpressing NtERF32, NtERF221, or NtMCY2a transcripts were treated with 0.1% DMSO (control) or 100 µM MeJA for 8 h before being collected for total RNA extraction. Transcript levels of NtAO, NtODC, NtPMT, NtQPT, and NtQS were measured by qRT-PCR, respectively. Expression level was represented as mean of relative expression values from three biological replicates (n = 3) normalized to NtEF-1α. Error bars indicate standard error. Colored asterisks indicate the statistical significance of the expression values relative to the wild-type controls with unpaired t-tests at p < 0.01.