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Expression of stemness markers in CCA cell lines. HuCCT1 and SNU1196 cells were cultured in six-well plates. Cells were harvested and total RNA was extracted at three different time intervals: 24, 48, and 72 h. Complementary DNA (cDNA) synthesis was performed from total RNA and used for real-time qPCR expression analysis. (a) mRNA expression of stemness markers (Nanog, Sox2, Oct4, and c-Myc) in HuCCT1 cells and (b) in SNU1196 cells.
