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. 2019 Oct 25;11(11):1651. doi: 10.3390/cancers11111651

Figure 1.

Figure 1

Transcriptome of motile and non-motile cells have major differences and SERPINE1 is the top upregulated gene in dispersive cells. (A) hanging drops method was used to generate tumor-mimicking spheroids. After formation of tumor spheroids in hanging drops, spheres were transferred to 24-well plates and allowed to disperse for 24 h. Core and dispersive cells were collected separately for RNA sequencing. (B) total 1627 genes were differentially expressed between motile and non-motile cells (log2 fold change -1≤ or ≥1, padj ≤ 0.05); (C) volcano plot showing the upregulated (red) and downregulated (blue) genes in dispersive cells; (D) qRT-PCR validation of top differentially expressed genes in core and dispersive cells; (E) “Diseases and bio functions” from IPA core functional analysis of the differentially expressed genes related to “cell movement” in the dispersive cells (z-score of >|2|); (F) top 15 cancer hallmark gene sets enriched in dispersive cell transcriptome in GSEA analysis (NOM p ≤ 0.05); (G) enrichment plot for EMT gene set; (H) gene expression heat map of EMT genes in core and dispersive cells (biological duplicates were shown).