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. 2019 Nov 5;10(11):890. doi: 10.3390/genes10110890

Table 1.

Primer sequences for PCR amplification of the chicken IL-6 gene.

Primer Primer Sequence (5′-3′) Length/bp Annealing Temperature/°C
P1 F: AGAGAGGACTAACCCACAGAG
R: CCAGCTTCTCCAGTCTTGTC
698 58.5
P2 F: AGGGACAGCAATGGCAGAAG
R: AAGAGCTGATCCTGGTTCTGG
717 60.5
P3 F: CAGAGGACGTCCTACCTCAA
R: GGTGAGCCTGGCAGCC
690 56.5
P4 F: AAGATAAGACGCGCCACACC
R: TTGAGGTTGTTCCGGACGAG
803 58.5