Table 1.
Primer | Primer Sequence (5′-3′) | Length/bp | Annealing Temperature/°C |
---|---|---|---|
P1 | F: AGAGAGGACTAACCCACAGAG R: CCAGCTTCTCCAGTCTTGTC |
698 | 58.5 |
P2 | F: AGGGACAGCAATGGCAGAAG R: AAGAGCTGATCCTGGTTCTGG |
717 | 60.5 |
P3 | F: CAGAGGACGTCCTACCTCAA R: GGTGAGCCTGGCAGCC |
690 | 56.5 |
P4 | F: AAGATAAGACGCGCCACACC R: TTGAGGTTGTTCCGGACGAG |
803 | 58.5 |