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Pairwise variations were calculated using the geNorm algorithm to determine the optimal numbers of reference genes needed for accurate RT-qPCR data normalization. The pairwise variation (Vn/Vn+1) was calculated using data from different developmental stages, different tissues, RBSDV-NV, RBSDV-V, or RSV-V L. striatellus, LsSYNJ1-silenced L. striatellus, and L. striatellus in all tested samples.
