Skip to main content
. 2019 Nov 1;11(11):1701. doi: 10.3390/cancers11111701

Figure 6.

Figure 6

The V658F mutation does not affect JAK1 binding to the box1/2 motif of a cytokine receptor. (A–C), Representative ITC curves of IFNλR peptide binding to JAK1 constructs (A) full-length JAK1; (B) full-length JAK1V658F; (C) JAK1 FERM-SH2 domains only. (D), Summary of IFNλR-JAK1 Kd values obtained from ITC experiments. No significant difference in IFNλR affinity was seen between full-length wild type, full-length V658F, nor the FERM-SH2 domains of JAK1. Error bars represent the standard error of the mean of three independent experiments (n = 3).