Table 5.
RT qPCR gene expression represented as average fold change evaluating the effect of stimulation in cell cultures without glutamate (ES_No-Glu).
| Biological Process | Gene | ES_No-Glu vs. No-ES_No-Glu | ||||||
|---|---|---|---|---|---|---|---|---|
| Monophasic Cathodic | AsymBi 1:0.5 | SymBi 1:1 | Cathodic PR | Anodic PR | AsymBi 1:2 | Monophasic Anodic | ||
| Immune Function | Gfap | 0.821 | 0.745 | 0.802 | 1.469 | 1.653 | 0.488 * | 0.700 |
| Synaptic Transmission | Slc7a11 | 0.914 | 0.690 | 0.596 | 1.204 | 1.924 ‡ | 0.843 | 0.921 |
| Glul | 0.659 | 1.469 | 1.068 | 1.111 | 1.108 | 0.724 | 0.580 ‡ | |
| Neuroprotection | S100a4 | 0.632 ‡ | 1.072 | 0.923 | 1.162 | 1.468 | 0.617 ‡ | 0.449 * |
| Redox Processes | Mt2a | 0.521 | 1.310 | 1.392 | 2.445 ‡ | 2.875 * | 0.722 | 0.444 |
| Gsr | 0.875 | 0.654 | 1.134 | 1.179 | 1.106 | 0.807 | 0.980 | |
| Hmox1 | 1.342 | 0.679 | 0.625 | 0.712 | 1.136 | 0.701 | 2.029 | |
| Cell Adaptive Response | Bag3 | 0.948 | 0.939 | 0.880 | 1.102 | 1.484 | 1.154 | 1.057 |
Anodic content in the waveform increases from left to right. p < 0.05 was considered significant. *, represents significance vs no-stimulation no-glutamate (No-ES_No-Glu); ‡ denotes 0.05 ≤ p < 0.10.