Table 6.
RT qPCR gene expression represented as average fold change evaluating effect of stimulation with glutamate (ES_Glu) relative to cells not exposed to ES and additional glutamate (No-ES_No-Glu).
| Biological Process | Gene | ES_Glu vs. No-ES_No-Glu | ||||||
|---|---|---|---|---|---|---|---|---|
| Monophasic Cathodic | AsymBi 1:0.5 | SymBi 1:1 | Cathodic PR | Anodic PR | AsymBi 1:2 | Monophasic Anodic | ||
| Immune Function | Gfap | 1.085 | 2.442 * | 1.061 | 4.184 * | 1.864 ‡ | 0.513 ‡ | 0.698 |
| Synaptic Transmission | Slc7a11 | 6.502 * | 3.170 * | 2.653 * | 7.083 * | 5.443 * | 2.592 * | 4.545 * |
| Glul | 1.015 | 0.991 | 0.848 | 1.352 | 1.600 | 1.034 | 0.846 | |
| Neuroprotection | S100a4 | 0.563 | 0.975 | 0.883 | 0.935 | 1.118 | 0.798 | 0.883 |
| Redox Processes | Mt2a | 2.129 | 2.855 * | 2.095 | 4.627 * | 2.983 * | 0.845 | 1.643 |
| Gsr | 0.886 | 1.017 | 1.058 | 0.807 | 1.377 | 0.724 | 1.065 | |
| Hmox1 | 2.830 * | 0.798 | 2.923 * | 1.750 | 3.804 * | 2.246 * | 3.574 * | |
| Cell Adaptive Response | Bag3 | 0.897 | 0.781 | 1.057 | 1.325 | 0.986 | 0.862 | 1.437 |
Anodic content in the waveform increases from left to right. p < 0.05 was considered significant. * represents significance vs. no-stimulation no-glutamate (No-ES No-Glu) ‡ denotes 0.05 ≤ p < 0.075.