Figure 3. Effect of synthetic analogs of 1-TbAd on lysosomal pH in THP-1 cells and Macrophages.

a) Synthetic 1-TbAd and analogs that differ in the linkage or nature of the lipid or carbohydrate were prepared as described in Supplementary Fig. 4. b) Phagocytosis of green fluorescent beads and the pH-sensitive lysotracker dye in human THP-1 cells was monitored by confocal microscopy in three independent experiments,, each evaluating 5 low power sections with all outcomes shown in Supplementary Figs. 5–6. P values were calculated with the two-tailed Student’s T test and standard deviations are shown. c-d) Carboxyfluorescein beads were added to murine bone marrow-derived macrophages seeded in a 96-well plate, or to wells containing only media, and fluorescence was tracked with a microplate reader every 2 minutes for 2 hours. Compounds at indicated concentrations were added at the start of the assay. Data are shown as means with standard deviations from 5 or 6 replicate wells, which are representative of three independent experiments.