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. 2019 Nov 20;21(1):35–42. doi: 10.3892/mmr.2019.10836

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Copyright: © Sun et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — miR-542-3p represses OTUB1 expression in esophageal cancer cells. (A) Transfection with miR-542-3p mimic successfully increased miR-542-3p expression in KYSE150 cells. (B and C) Overexpression of miR-542-3p decreased (B) OTUB1 mRNA and (C) OTUB1 and Snail protein expression in KYSE150 cells. (D) Transfection of miR-542-3p inhibitor successfully decreased miR-542-3p expression in KYSE150 cells. (E and F) Downregulation of miR-542-3p increased (E) OTUB1 mRNA and (F) OTUB1 and Snail protein expression in KYSE150 cells. *P<0.05, **P<0.01 and ***P<0.0001. miR-542-3p, microRNA-542-3p; NC, negative control; OTUB1, ovarian tumor domain-containing ubiquitin aldehyde-binding protein 1.