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. 2019 Nov 20;21(1):181–190. doi: 10.3892/mmr.2019.10834

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Confocal fluorescence images of cells for evaluation of Cy3-labeled siRNA internalization. LX-2 cells were transfected with PEI-Fe₃O₄/siRNA or Lipo 2000/siRNA. The cell nuclei are stained in blue, and siRNA in red (original magnification, ×1,200). The scale bar represents 10 µm. siRNA, small interfering RNA; PEI-Fe₃O₄, polyethyleneimine-functionalized magnetic iron oxide; cy3, cyanine 3; Lipo 2000, Lipofectamine 2000.