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. 2019 Nov 20;21(1):181–190. doi: 10.3892/mmr.2019.10834

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Effect of TGF-β1 on the activation of LX-2 cells. (A) Representative immunocytochemistry of α-SMA in LX-2 cells before or after treatment. The brown color indicates α-SMA staining (original magnification, ×400). The scale bar represents 50 µm. (B) Western blotting showed that the expression of CTGF protein was significantly increased in activated cells, compared with that in quiescent LX-2 cells. *P<0.05 vs. TGF-β1-non-treated cells. CTGF; connective tissue growth factor; TGF-β1, transforming growth factor-β1; α-SMA, α-smooth muscle actin.