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. Author manuscript; available in PMC: 2019 Dec 6.
Published in final edited form as: J Neurophysiol. 2008 Mar 5;99(5):2443–2455. doi: 10.1152/jn.00762.2007

FIG. 1.

FIG. 1.

Chronic MK-801 treatment in vivo increases the number of cholinergic neurons in the rat hypothalamus. A–D: representative images of the double immunostaining for NeuN (A, C) and ChAT (B, D) in the area dorsolateral to the SON in postnatal day 32 (P32) control (A, B) and MK-801–treated (C, D) rats are shown. The pairs of images (A and B; C and D) were taken from the same fields/sections. E and F: graphs represent statistical analysis for NeuN (E) and ChAT (F) expression (Student’s t-test; n = 35, control, and n = 45, MK-801–treated, in both E and F). ChAT, choline acetyltransferase; MK-801, dizocilpine; NeuN, a neuron-specific nuclear protein; SON, supraoptic nucleus.