TABLE 1.
Culture Condition | Number of Neurons p.m.f. |
Percentage of ChAT-Positive Neurons p.m.f. |
---|---|---|
A. Mouse (DIV4-DIV18 treatment) | ||
Control | 39.8 ± 1.3 | 1.3 ± 0.3 |
AP5 + CNQX | 43.9 ± 1.6 | 30.8 ± 2.4, *P < 0.001 |
AP5 | 41.3 ± 1.4 | 24.3 ± 2.0, *P < 0.001, #P < 0.05 |
NMDAR1 knockout | 42.4 ± 1.2 | 24.1 ± 1.8, *P < 0.001, #P < 0.05 |
B. Rat (DIV4-DIV18 treatment) | ||
Control | 45.2 ± 1.9 | 1.8 ± 0.4 |
AP5 + CNQX | 48.7 ± 2.0 | 30.7 ± 2.3, *P < 0.001 |
AP5 + CNQX + atropine + mecamylamine | 44.7 ± 2.9 | 2.5 ± 0.4, #P < 0.001 |
AP5 | 46.1 ± 1.9 | 22.8 ± 2.4, *P < 0.001, #P < 0.05 |
MK-801 | 47.0 ± 1.7 | 14.1 ± 2.8, *P < 0.001, #P< 0.001 |
Values are means ± SE. p.m.f., per microscope field. In each group, calculations were done in 10 microscope fields from three independent coverslips (n = 30 microscope fields; ×20 objective). Significance of differences (ANOVA with post hoc Tukey test) was calculated relative to the control (*) and AP5 + CNQX-treated (#) groups.