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. Author manuscript; available in PMC: 2019 Dec 6.
Published in final edited form as: J Neurophysiol. 2008 Mar 5;99(5):2443–2455. doi: 10.1152/jn.00762.2007

TABLE 1.

Expression of cholinergic neurons in hypothalamic cultures

Culture Condition Number of
Neurons
p.m.f.		 Percentage of ChAT-Positive
Neurons p.m.f.
A. Mouse (DIV4-DIV18 treatment)
Control 39.8 ± 1.3   1.3 ± 0.3
AP5 + CNQX 43.9 ± 1.6 30.8 ± 2.4, *P < 0.001
AP5 41.3 ± 1.4 24.3 ± 2.0, *P < 0.001, #P < 0.05
NMDAR1 knockout 42.4 ± 1.2 24.1 ± 1.8, *P < 0.001, #P < 0.05
B. Rat (DIV4-DIV18 treatment)
Control 45.2 ± 1.9   1.8 ± 0.4
AP5 + CNQX 48.7 ± 2.0 30.7 ± 2.3, *P < 0.001
AP5 + CNQX + atropine + mecamylamine 44.7 ± 2.9   2.5 ± 0.4, #P < 0.001
AP5 46.1 ± 1.9 22.8 ± 2.4, *P < 0.001, #P < 0.05
MK-801 47.0 ± 1.7 14.1 ± 2.8, *P < 0.001, #P< 0.001

Values are means ± SE. p.m.f., per microscope field. In each group, calculations were done in 10 microscope fields from three independent coverslips (n = 30 microscope fields; ×20 objective). Significance of differences (ANOVA with post hoc Tukey test) was calculated relative to the control (*) and AP5 + CNQX-treated (#) groups.