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. Author manuscript; available in PMC: 2019 Dec 6.
Published in final edited form as: Cell Rep. 2019 Oct 1;29(1):62–75.e7. doi: 10.1016/j.celrep.2019.08.073

Figure 4. Effects of AGX51 on HUVEC Growth.

Figure 4.

(A) Cell growth of HUVECs treated with DMSO or 20 μM AGX51 for 5 days.

(B) Cell cycle analysis of HUVECs treated with DMSO or 20 μM AGX51 for 24 h.

(C) Western blot for Cyclin D1 on whole-cell lysates from HUVECs treated with 0–40 μM AGX51 for 24 h. Tubulin is used as a protein loading control. See also Figure S5.

(D) HUVEC branching was observed after 18–20 h of culturing on matrigel in the absence or presence of 0–40 μM AGX51; images were taken at 10× magnification.

(E) Quantification of the number of nodes, junctions, meshes, and total branching length (n = 4 replicates per concentration tested); *p < 0.05 by Wilcoxon test.

(F) HUVEC monolayers were scratched, then media were replaced with media containing 0–40 μM AGX51, and migration was observed after 24 h, with images taken at 20× magnification.