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. 2019 Dec 5;38:482. doi: 10.1186/s13046-019-1481-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — NF-YAx attenuates HEK-293, SH-SY5Y and ST14A colony formation. a Representative Micrographs (bar = 100 μm) plus histograms, demonstrating Necrostatin-1 (Nec-1) (100 μM) but not z-VAD-fmk (10 μM) inhibition of NF-YAx-induced SH-SY5Y and HEK-293 cell-death. Histogram data are displayed as mean (± SD) percent adherent/alive and suspension/dead cells, in three independent assays performed in duplicate (* = significant). b Representative colony formation assays plus histograms, demonstrating inhibition of HEK293, SH-SY5Y and ST14A colony-formation by NF-YAx and NF-YAdn compared to NF-YAl, NF-YAs and pcDNA expression vectors. Histogram data are displayed as mean (± SD) percentage difference to pcDNA-transfected controls, in three independent assays performed in triplicate (* = significant)