Fig. 8.
NF-YAx selects neuroblastoma CSCs. a Representative RT-PCRs and histograms demonstrating differences in stem cell-associated CD133, Sox-2, CD117, Nestin, Nanog, p75NTR and Bmi1 expression and apoptosis/related EglN3, KIF1Bβ, Bcl2, Mcl1, BclxL, PUMA, BAD and BAX expression plus NF-YAl, NF-YAs, NF-YAx, GAP and 18S rRNA levels in stable pcDNA, NF-YAl, NF-YAs, NF-YAx1 and x2-transfectants. Histogram data are displayed as mean (± SD) fold densitometric difference compared to pcDNA control transfectants, in three independent experiments performed in duplicate. b RT-PCR comparing p75NTR, Bmi1 and 18S rRNA levels in parental SH-SY5Y and stable NF-YAx1 transfected SH-SY5Y cells. c Western blots demonstrating EglN3 and β-actin but not KIF1Bβ expression in pcDNA, NF-YAl, NF-YAs, NF-YAx1 and x2-transfectant extracts (30 μg). d RT-PCRs demonstrating Bmi1, p75NTR, CD133, Sox-2, CD117, Nestin, Nanog, NF-YAl, NF-YAs, NF-YAx and GAP expression levels in selected xenograft tumours formed by pcDNA, NF-YAl, NF-YAs, NF-YAx1 and NF-YAx2-transfectants