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. 2019 Nov 22;21(1):61–68. doi: 10.3892/mmr.2019.10843

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Copyright: © Fu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effects of LDN-212854 on the activation of BMP/SMAD signaling in KR-12-a6-treated hBMSCs. hBMSCs were divided into four treatment groups as follows: Control, KR-12-a6, LDN-212854, and KR-12-a6+LDN-212854. (A) Western blot analysis of the protein expression of p-Smad1/5 and Smad1/5 in the different treatment groups. β-actin served as the loading control. (B) Quantitative analysis of Smad1/5 phosphorylation. Data are presented as the mean ± SD (n=4). **P<0.01 vs. KR-12-a6 at 40 µg/ml without LDN-212854. hBMSC, human bone marrow mesenchymal stem cell; p, phosphorylated.