Table I.

Primer sequences for reverse transcription-quantitative PCR.

Gene	Forward primer	Reverse primer
RUNX2	5′-GCCACCACTCACTACCACACCTA-3′	5′-TCCTGACGAAGTGCCATAGTAGAGATAT-3′
ALP	5′-GGACCATTCCCACGTCTTCAC-3′	5′-CCTTGTAGCCAGGCCCATTG-3′
COL1A1	5′-TGGGAGGAAGCAAAAGACTC-3′	5′-GGGTCATTTCCACATGCTTT-3′
BSP	5′-TGCCTTGAGCCTGCTTCC-3′	5′-GCAAAATTAAAGCAGTCTTCATTTTG-3′
BMP2	5′-AACACTGTGCGCAGCTTCC-3′	5′-CTCCGGGTTGTTTTCCCAC-3′
OSX	5′-CCCCACCTCTTGCAACCA-3′	5′-CCCCACCTATTGCAACCA-3′
OPN	5′-GCCGACCAAGGAAAACTCACT-3′	5′-GGCACAGGTGATGCCTAGGA-3′
OCN	5′-CCCAGGCGCTACCTGTATCAA-3′	5′-GGTCAGCCAACTCGTCACAGTC-3′
β-actin	5′-TGGCACCCAGCACAATGAA-3′	5′-CTAAGTCATAGTCCGCCTAGAAGCA-3′

ALP, alkaline phosphatase; BMP, bone morphogenic protein; BSP, bone sialoprotein; COL1A1, type 1 collagen α1 chain; OCN, osteocalcin; OPN, osteopontin; OSX, osterix; RUNX2, runt-related transcription factor 2.