Effect of IGRS on cerebral ischemia-reperfusion injury. At 24 h post-reperfusion, rats were anesthetized by intraperitoneal injection with 10% chloral hydrate (350 mg/kg body weight) prior to sacrifice. Neurological scores were performed, and ischemic infarction volumes and brain water content were measured. (A) Representative images of TTC staining and a cerebral infarction volume histogram in coronal brain sections (n=6 per group). (B) Histogram of neurologic scores (n=6 per group). (C) Histogram of brain water content (n=6 per group). Values are presented as mean ± standard deviation of each group. ##P<0.01 vs. Sham group. *P<0.05 and **P<0.01 vs. Model group. TTC, 2,3,5-triphenyltetrazolium chloride; IGRS, iridoid glycosides from Radix Scrophulariae.