Fig. 6.

Metformin reverses the reduced PTPRD expression in cancer cells. a Protein and mRNA expression levels of PTPRD were assessed by western blotting and RT/qRT-PCR, respectively, upon metformin treatment of MKN74 and SNU216 cells with stable PTPRD knockdown. b Protein and mRNA expression levels of PTPRD were assessed by western blotting and RT−/qRT-PCR, respectively, upon metformin treatment of KATOIII, GCIY, and SNU668 cells, in which PTPRD expression is relatively downregulated. These data are representative of three independent experiments. **p < 0.01; ***p < 0.001 by unpaired Student’s t-test