Fig. 5.

Induction of HEXIM1 expression and inhibition of proliferation of TNBC subtypes by KDM5B inhibitors. a Left panel: TNBC lines were treated with DMSO or indicated concentrations of KDOAM25 for 6 days and subjected to MTT assays to assess proliferation. Right panel: TNBC lines were treated with DMSO or KDOAM25 (representing IC50) for 2 weeks and subjected to colony formation assays. b Different breast cell lines were transfected or treated with DMSO, 4a1 (100 μM), or KDOAM25 (500 nM) for 10 h and processed for western blot analyses of indicated proteins or subjected to MTT assays to assess proliferation. Figures are representative of at least three experiments. Upper panel: *P < 0.01 relative to DMSO-treated control of the same cell line based on t test. Lower panel: *P < 0.01 relative to cells of the same cell line transfected with control vector based on t test