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. 2019 Dec 5;21:138. doi: 10.1186/s13058-019-1228-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — In vivo testing of KDOAM25 activity. PLGA or PLGA-KDOAM25 (1 μM, 50 μl volume) were injected into mammary glands once/week for 4 weeks. a Body weights were monitored weekly. b Blood cell levels were determined using the HEMAVET 950FS. c Western blot analyses of indicated proteins in the mammary gland. d Final tumor weights and e upper left panel: lungs from PLGA ± KDOAM25-treated PyMT mice. Upper right panel: quantification of tumor area in hematoxylin and eosin-stained lung tissues. Lower left panel: number of metastatic lesions visible in hematoxylin and eosin-stained lung tissues in the lungs of each mouse. Panels represent 5–6 mice per group (PLGA ± KDOAM25). *P < 0.05 relative to PLGA-treated mice based on t test