Figure 2.
Prediction of tumor purity based on immunofluorescence-based image analysis for gastric PDCs (A) Conditions of the various heterogenous cell lines combining NCI-N87 cancer cells with fibroblasts and the fluorescence intensity imaging results. Increased fluorescence intensity of EpCAM and decreased fluorescence intensity of vimentin with increasing concentrations of actual tumor content. Actual tumor purity in mixed cancer cells is calculated as the ratio of EpCAM intensity to combining EpCAM and vimentin intensity. (B) From this ratio, the predicted model of tumor purity in PDCs was derived, and the correlation R2 was 0·9373. (C) Drug response curve with lapatinib and various conditions of actual tumor purity in mixed cells.