Table 1.

Overview of histopathological findings of mice subcutaneously infected with autoluminescent Mycobacterium ulcerans into the tail

Clinical picture	Identifier	Bioluminescence (in photons/s) area under the curve	Acid-fast bacilli	Inflammation	Tissue damage	Vascular involvement	Inflammatory cell type	Degree of inflammation
Severe pathology (sacrificed at week 8)	84	1.36E+08	++	+++	+++	n/a	PC, MΦ, and LYM	Severe, multifocal, and chronic
	85	1.35E+08	+++	+++	+++	n/a	PC, MΦ, LYM, and EOS	Severe, multifocal, and chronic
	88	1.72E+07	++	+++	+++	++	PC, MΦ, and LYM	Severe, multifocal, and chronic
Moderate pathology	86	1.97E+06	−	+	+	+	MΦ and LYM	Mild to moderate, chronic, and multifocal
	89	3.69E+06	+	+/++	++	++	PC and MΦ	Mild, diffuse, and chronic
No macropathology	81	2.15E+06	−	+	+	−	MΦ and LYM	Moderate, chronic, and focal
	82	692641	−	−	−	−	−	None
	83	872438	−	+	+	+	MΦ and LYM	Moderate, chronic, and multifocal
	87	7.24E+06	++	+++	+++	++	PC, MΦ, and LYM	Locally severe and chronic
	90	0	−	+	+	+	PC, MΦ, EOS, and NEU	Mild, chronic, and multifocal

EOS = eosinophil; LYM = lymphocyte; MΦ = macrophage; NEU = neutrophil; PC = plasma cell; not applicable. Animals were divided by clinical pathology in severe pathology, moderate pathology, and no marcopathology. Photons per second analyzed by IVIS imaging are shown in comparison with histological results. The amount of photos/second as a proxy for bacterial quantity correlated with pathology, except in mouse 87, where no clinical pathology was seen.