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. 2019 Dec 2;12:10547–10558. doi: 10.2147/OTT.S225918

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© 2019 Ding et al.

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DHA induces apoptosis in PC-9 and PC-9/GR cells. (A–C) Inhibition rate of the two different cell lines (PC-9, PC-9/GR) was assessed after 24 hrs of gefitinib stimulation by CCK8 assay. (D–F) Inhibition rate of the two different cell lines (PC-9, PC-9/GR) was assessed after 24 hrs of DHA stimulation by CCK8 assay. (G–H) Both PC-9 and PC-9/GR cells were seeded in 6-well plates, after DHA (50μM) treatment for 24hrs, both PC-9 and PC-9/GR cells were stained with Annexin V and PI and apoptosis cells were quantitated by flow cytometer. Results from the experiments are shown as means ± SEM. The data are presented as the percentage of cell inhibition rate to unstimulated cells (0μM). ***p<0.001 versus non-treated control of PC-9 and PC-9/GR cells.