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. 2019 Dec 2;12:10515–10522. doi: 10.2147/OTT.S215998

Figure 2.

Figure 2

miR-509-5p inhibits proliferation and induces apoptosis of testicular germ cell tumor cells. (A-C) TGCT cell lines NT2 and NCCIT were transfected with negative control mimic (NC mimic) or 100 nM miR-509-5p mimic. After 3 days, cells were harvested for following analyses. (A) miR-509-5p level was determined by qRT-PCR analysis (n = 3). U6 snRNA level was used as an internal control. (B) Cell proliferation was assessed using CCK-8 assay (n = 5). (C) Cell apoptosis was analyzed by FACS (n = 5). (D) NT2 and NCCIT were transfected with NC mimic, 50 mM or 100 nM miR-509-5p mimic. After 3 days, the cleaved caspases 3 was detected by Western blotting. (E-H) NT2 and NCCIT were transfected with negative control inhibitor (NC inhibitor) or miR-509-5p inhibitor. After 3 days, the miR-509-5p level (E), cell proliferation (F), and cell apoptosis (G-H) were analyzed as in (A-D). Data are mean ± SD. **P < 0.01.