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. 2019 Dec 2;12:10515–10522. doi: 10.2147/OTT.S215998

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© 2019 Sun et al.

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miR-509-5p targets MDM2. (A, upper panel) The putative binding sites for miR-509-5p in MDM2 3ʹ-UTR was predicted by TargetScan algorithms. (A, bottom panel) NC mimic or miR-509-5p mimic was cotransfected with luciferase reporter construct with either wild-type (wt-MDM2) or mutant MDM2 3ʹ-UTR (mut-MDM2) into HEK293 cells. The luciferase activity assayed at 36 h after transfection (n = 5). (B-D) NT2 and NCCIT were transfected with NC mimic or miR-509-5p mimic, or NC inhibitor or miR-509-5p inhibitor. The mRNA level (B-C) and protein level (D) of MDM2 were determined by qRT-PCR analysis and Western blotting analysis, respectively. (E-F) The mRNA level (E) and protein level (F) of MDM2 in the testes of normal controls (NC, n = 8) and infertile men with maturation arrest (MA, n = 12) were determined. Data are mean ± SD. **P < 0.01; NS, not significant.