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. 2019 Dec 2;12:10515–10522. doi: 10.2147/OTT.S215998

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© 2019 Sun et al.

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MDM2 restoration rescues miR-509-5p effects on testicular germ cell tumor cells. (A-C) NT2 cells were transfected with NC mimic or 100 nM miR-509-5p mimic in combination with pcDNA3.1-vector or pcDNA3.1-MDM2. (A) The protein level of MDM2 was determined by Western blotting analysis. (B) Cell proliferation was determined by CCK-8 method (n = 5). (C) Cell apoptosis was assessed by FACS analysis (n = 5). (D-F) NCCIT cells were treated as in (A). The protein level of MDM2 (D), cell proliferation (E), and cell apoptosis (F) were analyzed as in (A-C). Data are mean ± SD. *P < 0.05; **P < 0.01.