Figure 5.
Overexpression of JAZF1 inhibits TAK1 and NF-κB p-p65 expression and inhibits NF-κB p65 nuclear translocation in BCPAP cells. (A) WB analysis of the protein levels of TAK1 and NF-κB p65 in response to JAZF1 overexpression. ***P<0.001. (B) RT-qPCR analysis of the mRNA levels of NF-κB p65. (C) Protein levels of NF-κB p-p65 at different times in response to JAZF1 overexpression. ***P<0.001. (D) Detection of NF-κB p65 subcellular localization, red indicates NF-κB p65 and blue indicates nuclei.