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. 2019 Nov 29;13:531. doi: 10.3389/fncel.2019.00531

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Copyright © 2019 Plastira, Joshi, Bernhart, Schoene, Specker, Nazare and Sattler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AS2717638 and compound 3 reduce LPA-mediated secretion of pro-inflammatory cytokines and chemokines. BV-2 microglia were cultured on 24-well plates and serum-starved o/n. The supernatants were collected after incubation with vehicle control (DMSO), DMSO plus LPA (1 μM) or LPA plus AS2717638 (0.1 μM) or compound 3 (1 μM) for 2, 8, and 24 h. ELISA was used to quantify the concentrations of IL-6, TNFα, IL-1β, CXCL10 (IP-10), CXCL2 (MIP-2), and CCL5 (RANTES). Results shown represent mean ± SD from three independent experiments performed in triplicate (*p < 0.05; **p < 0.01 compared to vehicle control; ^#p < 0.05, ^##p < 0.01; each inhibitor compared to LPA-treated cells; one-way ANOVA with Bonferroni correction). No bars = below detection limit.