TABLE 2.

List of the oligonucleotide primers used for RT-PCR and RT-qPCR.

Gene Symbol	Primer Sequence	RefSeq Accession	Annealing Temp (°C)	PCR Product Size (bp)
Actb	Forward: 5’ CCAGATCATGTTTGAGACCTTCAA 3’ Reverse: 5’ CCAGAGGCATACAGGGACAGC 3’	NM_007393.5	61	78
Ar	Forward: 5’ AGAATCCCACATCCTGCTCAA 3’ Reverse: 5’ AAGTCCACGCTCACCATATGG 3’	NM_013476.4	58	133
Cyp19a1	Forward: 5’ ACCTCGGGCTACGTGGATGTG 3’ Reverse: 5’ GAT GTTT GGTTT GAT GAGGAGAGC 3’	NM_007810.4	60	159
Dhx8	Forward: 5’ AATCCAGAGAACCAACCTGGC 3’ Reverse: 5’ GATCAGAGTTTCCATCGGTGG 3’	NM_144831.2	60	109
Dusp11	Forward: 5’ AACCAGCATTATGGCCGACAT 3’ Reverse: 5’ GGAGAT AGTCTTT CCACCTTT CG 3’	NM_028099.4	58	112
Elav14	Forward: 5’ CCAATCACCATTGACGGGATG 3’ Reverse: 5’ CAGGGGACAGGTT AT AGACGAA 3’	NM_010488.4	60	97
Esr1	Forward: 5’ CACTTT GATCCACCT GAT GGC 3’ Reverse: 5’ GGCACAACGTTCTTGCATTTC 3’	NM_007956.5	58	153
Esr2	Forward: 5’ GAAAGTGACGCGATACACCGT 3’ Reverse: 5’ CCT GT GAGGTAGGAAT GCGAA 3’	NM_010157.3	58	150
Khdrbs2	Forward: 5’ GGGGT GAATAT GAT GACCAGACC 3’ Reverse: 5’ CCT CGTTTACT CCAT GACCGT AG 3’	NM_133235.3	59	105
Nanos2	Forward: 5’ CATGGACCGTGTGAGTCTTG 3’ Reverse: 5’ TTCTTGGCAGTGACGATCAG 3’	NM_194064.2	58	197
Ncbp2	Forward: 5’ T GT ACT GGAAGCCACCT CT G 3’ Reverse: 5’ TGTTGCCTGGGTAGAGCTTT 3’	NM_026554.4	58	126
Ppargc1b	Forward: 5’ TCCTGTAAAAGCCCGGAGTAT 3’ Reverse: 5’ GCT CT GGTAGGGGCAGT GA 3’	NM_133249.2	58	174
Rbm12	Forward: 5’ GTT AT GCCTT CGGTT GGAAA 3’ Reverse: 5’ GGCCATTT CCAAAGTT CT GA 3’	NM_029397.3	55	130
Rbm48	Forward: 5’ AAACCTGGTGGCGTATTCGAT 3’ Reverse: 5’ ACGCT CAACTAATT CCTT CAT GG 3’	NM_172991.4	58	177
Rpl13a	Forward: 5’ ATGAGGTCGGGTGGAAGTACC 3’ Reverse: 5’ CAGGAGT CCGTT GGT CTT GAG 3’	NM_009438.5	62	179
Sfpq	Forward: 5’ GGAGTTCCACCAGCAACCAT 3’ Reverse: 5’ CTGCCCAAAGCGCTCAGT 3’	NM_023603.3	60	69
Srsf3	Forward: 5’ TGAGGATCCCCGAGATGCT 3’ Reverse: 5’ CTTACACGGCAGCCACACAGT 3’	NM_013663.5	60	72
Srsf10	Forward: 5’ GCGT CAGATT GAAAT CCAGTT CG 3’ Reverse: 5’ CGAGAGCGTCTAT AT CGGT CAT 3’	NM_010178.3	60	126
Strbp	Forward: 5’ GT GT GGT GTAAT GAGGATT GG 3’ Reverse: 5’ TTTCCGTGGGTTTGTCTTTGC 3’	NM_009261.4	58	101
Tdrd3	Forward: 5’ AGTGACATCAACGGTGGAAAG 3’ Reverse: 5’ GCTTGCGATTCCTCGTTATCCT 3’	NM_001253755.1	58	107
Thoc3	Forward: 5’ AT CT GGGATGT GAGGACT ACAA 3’ Reverse: 5’ T GT GT CTT GGCGT CAAT GAAA 3’	NM_028597.3	59	143
Tut1	Forward: 5’ GATT CGGACGTT GT AT CGCT G 3’ Reverse: 5’ CTCGGAGTTGCACCAAATCC 3’	NM_197993.3	59	130
Xist	Forward: 5’ GCT GGTT CGT CTAT CTT GT GGG 3’ Reverse: 5’ GGAT CCT GCACT GGAT GAGT 3’	NR_001463.3	58	225