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Dilution metrics to obtain optimum drug concentration for mitochondrial stress test in each well of the analyzer.
| Port | Drug | Final well concentration (μM) |
Stock solution volume (μL) |
Media volume (μL) |
Port solution (μM) | Volume added to port (μL) |
|---|---|---|---|---|---|---|
| Port A | Oligomycin | 2 | 100 | 181.25 | 16 | 25 |
| Port B | FCCP | 1.5 | 100 | 270.4 | 13.5 | 25 |
| Port C | Rotenone/antimycin A | 0.5 | 60 | 240 | 5 | 25 |
