| Antibodies |
|
|
| Anti-mouse CD4 (GK1.5) |
BD Biosciences |
Cat# 552051; RRID: AB_394331 |
| Anti-mouse CD4 (RM4–5) |
Biolegend |
Cat# 100531; 100559; RRID: AB_493374; RRID:
AB_2562608 |
| Anti-mouse TCRβ (H57–597) |
Biolegend |
Cat# 109220; RRID: AB_893624 |
| Anti-mouse CD3 (145–2C11) |
Biolegend |
Cat# 100233; 100306; RRID: AB_2561387; RRID:
AB_312671 |
| Anti-mouse CD25 (PC61) |
BD Biosciences |
Cat# 553866; RRID: AB_395101 |
| Anti-mouse CD25 (PC61) |
Biolegend |
Cat# 102025; RRID: AB_830744 |
| Anti-mouse CD69 (H1.2F3) |
Biolegend |
Cat# 104512; RRID: AB_493564 |
| Anti-mouse GITR (DTA-1) |
Biolegend |
Cat# 126308; 126317; RRID: AB_1089125; RRID:
AB_2563385 |
| Anti-mouse CTLA-4 (UC10–4B9) |
Biolegend |
Cat# 106306; RRID: AB_313255 |
| Anti-mouse KLRG1 (2F1/KLRG1) |
Biolegend |
Cat# 138410; 138418; RRID: AB_10643582; RRID:
AB_2563015 |
| Anti-mouse Blimp1 (5E7) |
BD Biosciences |
Cat# 563643; RRID: AB_2738342 |
| Anti-mouse TIGIT (1G9) |
Biolegend |
Cat# 142103; RRID: AB_10895760 |
| Anti-mouse RORγt (B2D) |
eBioscience |
Cat# 12698182; RRID: AB_10807092 |
| Anti-mouse CCR6 (29–2L17) |
Biolegend |
Cat# 129807; RRID: AB_1227498 |
| Anti-mouse ST2 (RMST2–2) |
eBioscience |
Cat# 46933580; RRID: AB_2573882 |
| Anti-mouse ST2 (DIH9) |
Biolegend |
Cat# 145303; RRID: AB_2561914 |
| Anti-mouse Granzyme B (NGZB) |
eBioscience |
Cat# 11889882; RRID: AB_10733414 |
| Anti-mouse Granzyme B (GB11) |
Biolegend |
Cat# 515408; RRID: AB_2562196 |
| Anti-mouse IL-23R (12B2B64) |
Biolegend |
Cat# 150903; RRID: AB_2572188 |
| Anti-mouse Bcl2 (3F11) |
BD Biosciences |
Cat# 554221; RRID: AB_395312 |
| Anti-mouse Bim (C34C5) |
Cell Signaling Technology |
Cat# 12186; RRID: AB_2797842 |
| Anti-mouse Helios (22F6) |
Biolegend |
Cat# 137229; 137220; RRID: AB_2561639; RRID:
AB_10690535 |
| Anti-mouse Ki-67 (SolA15) |
eBioscience |
Cat# 12569882; RRID: AB_11150954 |
| Anti-mouse CD138 (281–2) |
BD Biosciences |
Cat# 553713; RRID: AB_394999 |
| Anti-mouse CD45.1 (A20) |
Biolegend |
Cat# 110728; RRID: AB_893346 |
| Anti-mouse CD45.2 (104) |
Biolegend |
Cat# 109837; RRID: AB_2561393 |
| Anti-mouse CD19 (1D3) |
BD Biosciences |
Cat# 551001; RRID: AB_394004 |
| Anti-mouse B220 (RA3–6B2) |
BD Biosciences |
Cat# 553090; RRID: AB_394620 |
| Anti-mouse B220 (RA3–6B2) |
eBioscience |
Cat# 25045282; RRID: AB_469627 |
| Anti-mouse CD44 (IM7) |
Biolegend |
Cat# 103028; RRID: AB_830785 |
| Anti-mouse CD62L (MEL-14) |
Biolegend |
Cat# 104441; RRID: AB_2561537 |
| Anti-mouse Fas (15A7) |
BD Biosciences |
Cat# 554258; RRID: AB_395330 |
| Anti-mouse IgM (II/41) |
eBioscience |
Cat# 11579081; RRID: AB_465244 |
| Anti-mouse T-/ B cell activation Ag (GL7) |
Biolegend |
Cat# 144606; RRID: AB_2562185 |
| Anti-mouse ICOS (C398.4A) |
Biolegend |
Cat# 313508; 313518; RRID: AB_416332; RRID:
AB_10641280 |
| Anti-mouse PD-1 (J43) |
BD Biosciences |
Cat# 551892; RRID: AB_394284 |
| Anti-mouse PD-1 (29F.1A12) |
Biolegend |
Cat# 135216; 135213; RRID: AB_10689635; RRID:
AB_10689633 |
| Anti-mouse IFN-γ (XMG1.2) |
BD Biosciences |
Cat# 554412; RRID: AB_395376 |
| Anti-mouse IL-10 (JES5–16E3) |
BD Biosciences |
Cat# 554467; RRID: AB_395412 |
| Anti-mouse IL-4 (11B11) |
Biolegend |
Cat# 504112; RRID: AB_493323 |
| Anti-mouse IL-17A (eBio17B7) |
eBioscience |
Cat# 50717782; RRID: AB_11220280 |
| Anti-Human/mouse Bcl6 (K112–91) |
BD Biosciences |
Cat# 561522; RRID: AB_10717126 |
| Anti-mouse FoxP3 (FJK-16 s) |
eBioscience |
Cat# 25577382; 45577382; RRID: AB_891552;
RRID: AB_914351 |
| Anti-mouse CXCR5 (2G8) |
BD Biosciences |
Cat# 551960; RRID: AB_394301 |
| Streptavidin-APC |
Biolegend |
Cat# 405207 |
| Streptavidin-APC.Cy7 |
Biolegend |
Cat# 405208 |
| Anti-mouse CD90.1 (OX-7) |
Biolegend |
Cat# 202535; RRID: AB_2562643 |
| Anti-mouse NK1.1 Biotin (PK136) |
Biolegend |
Cat# 108704; RRID: AB_313391 |
| Anti-mouse B220 Biotin (RA3–6B2) |
Biolegend |
Cat# 103204; RRID: AB_312989 |
| Anti-mouse CD4 Biotin (GK1.5) |
Biolegend |
Cat# 100404; RRID: AB_312689 |
| Anti-mouse CD8 Biotin (53–6.7) |
Biolegend |
Cat# 100704; RRID: AB_312743 |
| Anti-Human/mouse pSTAT5 (47/Stat5, pY694) |
BD Biosciences |
Cat# 612599; RRID: AB_399882 |
| Anti-Human/mouse pSTAT3 (4/P-STAT3,
pY705) |
BD Biosciences |
Cat# 612569; RRID: AB_399860 |
| Annexin-V |
BD Biosciences |
Cat# 561012; RRID: AB_2034024 |
| Anti-human IgG Fc (HP6017) |
Biolegend |
Cat# 409319; RRID: AB_2563329 |
| Goat anti-mouse IgG Fc HRP |
Invitrogen |
Cat# A16084; RRID: AB_2534758 |
| Rat anti-mouse IgG1 (X56) HRP |
BD Biosciences |
Cat# 559626; RRID: AB_397292 |
| Purified Anti-Mouse CD16/CD32 (Fc Block) |
BD Biosciences |
Cat# 553142; RRID: AB_394657 |
| Purified NA/LE anti-mouse CD3
(145–2C11) |
BD Biosciences |
Cat# 553057; RRID: AB_394590 |
| Purified NA/LE anti-mouse CD28 (37.51) |
BD Biosciences |
Cat# 553294; RRID: AB_394763 |
| AffiniPure F(ab’)2 Fragment
Goat anti-IgM |
Jackson Immunoresearch |
Cat# 115–006–020; RRID:
AB_2338469 |
| Purified anti-Blimp1 (3H2E8) |
Santa Cruz Biotechnology |
Cat# sc-66015; RRID: AB_1119615 |
| Purified anti-acetylated H3 (rabbit
polyclonal) |
Millipore |
Cat# 06–599; RRID: AB_2115283 |
| Rabbit IgG, polyclonal – Isotype
control |
Abcam |
Cat# ab-171870; RRID: AB_2687657 |
| Purified anti-H3K27me3 (mouse monoclonal) |
Abcam |
Cat# ab-6002; RRID: AB_1977539 |
| Mouse IgG isotype control |
Thermo Fisher Scientific |
Cat# 10400C; RRID: AB_2532980 |
| Monoclonal anti-β-actin-Peroxidase
(AC-15) |
Sigma |
Cat# A-3584; RRID: AB_2765165 |
| Hamster anti-mouse CD3ε
(eBio500A2) |
eBioscience |
Cat# 14–0033–85; RRID:
AB_837129 |
| Alexa Fluor 568 goat anti-hamster IgG |
Thermo Fisher Scientific |
Cat# A-21112; RRID: AB_2535761 |
| Alexa Fluor 647 rat anti-mouse IgD
(11–26c.2a) |
BioLegend |
Cat# 405708; RRID: AB_893528 |
| Rabbit anti-mouse Ki67 (polyclonal) |
Thermo Fisher Scientific |
Cat# PA5–19462; RRID: AB_10981523 |
| Alexa Fluor 488 goat anti-rabbit IgG |
Thermo Fisher Scientific |
Cat# A-11008; RRID: AB_143165 |
| Chemicals, Peptides, and
Recombinant Proteins |
| TransIT-LT1 |
Mirus |
Cat# MIR2300 |
| Tamoxifen |
Sigma |
Cat# T5648 |
| Sunflower seed Oil |
Sigma |
Cat# S5007 |
| Anti-mouse CD4 microbeads |
Miltenyi Biotec |
Cat# 130–049–201; RRID:
AB_2722753 |
| Anti-mouse CD19 microbeads |
Miltenyi Biotec |
Cat# 130–052–201 |
| Anti-biotin microbeads |
Miltenyi Biotec |
Cat# 130–090–485; RRID:
AB_244365 |
| CFA |
Sigma |
Cat# F5881 |
| IFA |
Sigma |
Cat# F5506 |
| Imject Alum |
Thermo Fisher Scientific |
Cat# 77161 |
| NP16-OVA (16 loading) |
Biosearch Technologies |
Cat# N-5051–100 |
| NP23-KLH (23 loading) |
Biosearch Technologies |
Cat# N-5060–25 |
| NP23-BSA (23 loading) |
Biosearch Technologies |
Cat# N-5050–10 |
| NP4-BSA (4 loading) |
Biosearch Technologies |
Cat# N-5050–10 |
| rhIL-2 |
Peprotech |
Cat# 200–02 |
| Mouse IL-21R Fc Chimera Protein |
R&D Systems |
Cat# 596-MR-100 |
| Leukocyte Activation cocktail |
BD Biosciences |
Cat# 550583 |
| Formaldehyde (16%, w/v), Methanol-free |
Pierce |
Cat# 28906 |
| Protein G Dynabeads |
Thermo Fisher Scientific |
Cat# 10003D |
| Bouin’s solution |
Sigma |
Cat# HT10132 |
| Critical Commercial Assays |
| FoxP3 staining Buffer Set |
eBioscience |
Cat# 00552300 |
| BD Cytofix/Cytoperm Solution kit |
BD Biosciences |
Cat# 554714 |
| Anti-nuclear Antibodies (ANA) Elisa kit |
Alpha Diagnostic |
Cat# 5210 |
| IgE OptEIA ELISA Set |
BD Biosciences |
Cat# 555248 |
| RNeasy plus micro kit |
QIAGEN |
Cat# 74034 |
| TaqMan RNA-to-CT™ 1-Step Kit |
Thermo Fisher Scientific |
Cat# 4392653 |
| ChIP-IT® Express Enzymatic |
Active Motif |
Cat# 53009 |
| Chromatin IP DNA Purification Kit |
Active Motif |
Cat# 58002 |
| Deposited Data |
| Microarray dataset |
This paper |
GEO: GSE101611
|
| Experimental Models: Cell
Lines |
| 293 T |
ATCC |
Cat# CRL-3216; RRID: CVCL_0063 |
| Experimental Models:
Organisms/Strains |
| Mouse: B6: C57BL/6J |
Jackson Laboratories |
Jax:000664; RRID: IMSR_JAX: 000664 |
| Mouse: Prdm1fl/fl:
B6.129-Prdm1tm1Clme/J |
Jackson Laboratories |
Jax:008100; RRID: IMSR_JAX:008100 |
| Mouse: FoxP3Cre:
B6.129(Cg)-Foxp3tm4(YFP/cre)Ayr/J
|
Jackson Laboratories |
Jax:016959; RRID: IMSR_JAX:016959 |
| Mouse: iCre+:
B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J |
Jackson Laboratories |
Jax:008463; RRID: IMSR_JAX:008463 |
| Mouse: Bcl6fl/fl:
B6.129S(FVB)-Bcl6tm1.1Dent/J |
Jackson Laboratories |
Jax:023727; RRID: IMSR_JAX:023727 |
| Mouse: Stat3fl/fl:
B6.129S1-Stat3tm1Xyfu/J |
Jackson Laboratories |
Jax:016923; RRID: IMSR_JAX:016923 |
| Mouse:
Tcrα−/−:
B6.129S2-Tcratm1Mom/J |
Jackson Laboratories |
Jax:002116; RRID: IMSR_JAX:002116 |
| Mouse: FoxP3-GFP:
B6.Cg-Foxp3tm1Mal/J |
Jackson Laboratories |
Jax:018628; RRID: IMSR_JAX:018628 |
| Mouse:
Rag2−/−:
B6.129S6-Rag2tm1Fwa N12 |
Taconic |
Model# RAGN12; RRID: IMSR_TAC:ragn12 |
| Mouse: B6SJL:
B6.SJL-Ptprca-BoyAiTac |
Taconic |
Model# 4007; RRID: IMSR_CMMR:PST4007 |
| Oligonucleotides |
| ChIP-PCR Il23r forward |
Integrated DNA Technologies |
CTTGGCAAACTTCCTTCCTATTAAC |
| ChIP-PCR Il23r reverse |
Integrated DNA Technologies |
AAACAGTGCTGACTACTT GGCAT |
| ChIP-PCR Il2ra forward |
Integrated DNA Technologies |
TCGGAGAGGGATTCGGTAGCTTGA |
| ChIP-PCR Il2ra reverse |
Integrated DNA Technologies |
TGATAGCCTGCTGCTCAGAACTGGG |
| ChIP-PCR Il2raCtrl
forward |
Integrated DNA Technologies |
TTACAGCAGTGCCTCCCTTG |
| ChIP-PCR Il2raCtrl
reverse |
Integrated DNA Technologies |
GGGAGTGAGTGGGGTTAGGA |
| ChIP-PCR Cxcr5 forward |
Integrated DNA Technologies |
GGGCAGGAAGAACAGAGTAAG |
| ChIP-PCR Cxcr5 reverse |
Integrated DNA Technologies |
CTGCTAACCACAGAGGAAGAC |
| ChIP-PCR Ccr7 forward |
Integrated DNA Technologies |
CACTCAAGCCAAGACAGCTA |
| ChIP-PCR Ccr7 reverse |
Integrated DNA Technologies |
GACTACTCAACCAGGGTGTTC |
| ChIP-PCR Ctla4 forward |
Integrated DNA Technologies |
AATATGTTTCTCTGCGGGCACCA |
| ChIP-PCR Ctla4 reverse |
Integrated DNA Technologies |
GCCTAAGTAAACCCCAGATCAGC |
| ChIP-PCR FoxP3 forward |
Integrated DNA Technologies |
CACCCTACCTGGGCCTATCC |
| ChIP-PCR FoxP3 reverse |
Integrated DNA Technologies |
GCTTCATCGGCAACAAGGAG |
| shRNA targeting sequence:
Il23r
|
Integrated DNA Technologies |
CCTACATAGATACCAAGTATA |
| TaqMan probe for Bcl6
|
Thermo Fisher Scientific |
Mm00477633_m1 |
| TaqMan probe for Prdm1
|
Thermo Fisher Scientific |
Mm00476128_m1 |
| TaqMan probe for Rps18
|
Thermo Fisher Scientific |
Mm02601777_g1 |
| Recombinant DNA |
| RV-pMIG-STAT5CA
|
Johnston et
al., 2012 |
N/A |
| pLKO.3 Thy1.1 |
Benoist and Mathis Lab (Harvard Medical
School) |
RRID: Addgene_14749 |
| PsPAX2 |
https://tronolab.epfl.ch |
RRID: Addgene_12260 |
| pCMV-VSV-G |
Stewart et
al., 2003 |
RRID: Addgene_8454 |
| Software and Algorithms |
| FlowJo |
FlowJo, LLC |
v. 10; RRID: SCR_008520 |
| FACSDiva |
BD Bioscience |
RRID: SCR_001456 |
| Prism 6 |
GraphPad |
v. 6; RRID: SCR_002798 |
| ImageJ |
NIH |
RRID: SCR_003070 |
| DAVID Bioinformatics Resources |
https://david.ncifcrf.gov |
v. 6.8; RRID: SCR_001881 |
| Ingenuity Pathway analysis (IPA) |
QIAGEN |
RRID: SCR_008653 |
