Fig 6. Mucinase activity of EatA is retained.

(A) Purified recombinant EatA degrades the synthetic protein Suc-Ala-Ala-Pro-Leu-p-anilimide in a colorimetric assay measuring the rate of release of p-anilimide. 2 independent experiments with 3 replicates total, medians with 95% CI are shown, (p = 0.1, Mann-Whitney U). (B) Degradation of MUC2 by recombinant EatA from H10407 and strain 700241. Purified EatA and MUC2 from LS174T cells (~540 kDa) were incubated together with mucin degradation (~300 kDa) assessed by western blotting, 2 independent experiments performed.