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. 2019 Dec 6;14(12):e0225137. doi: 10.1371/journal.pone.0225137

Fig 1. Sample collection, processing and RNA extraction: Whole blood was collected in triplicate followed by peripheral blood mononuclear cells (PBMCs) separation in a subset of samples.

Fig 1

The following sample collection tubes were used for the study: RNAgard®, PAXgene® RNA, EDTA, ACD-A, and CPT tubes. The PBMC separation was done using standard procedures for CPT tubes, and magnetic bead, LeukoLOCK™ and LSM methods. The samples were then stored at either 4°C (Cold) or -80°C (Frozen) and shipped overnight (o/n) for follow-up RNA extraction. Next, they were treated with one or more of several different RNA extraction procedures: Biomaxi Precip Buffer/ PAXgene® Blood miRNA, PAXgene® Blood miRNA, TRIzol® LS, ACK Lysing Buffer/ Qiagen miRNeasy, and TRIzol® Reagent manufacturer’s protocol.